Key Terms
Before preparing your samples for PhenoCycler (CODEX) imaging in the Enable lab, here is some terminology as used by our team:
- Block
- A formalin-fixed, paraffin-embedded (FFPE) or fresh frozen tissue specimen, ready to be sectioned and mounted
- Coverslip
- Treated 22x22mm cover glass; for mounting samples to be imaged on Keyence microscope
- Prepped and provided by Enable Medicine
- Replicate
- Serial sections of any sample or sample array, mounted for Fusion microscope
- Preventative against breakage or need to re-run experiments
- Sample
- A uniquely identified tissue specimen
- Sample ID
- The unique identifier for a given sample
- Slide
- A standard, charged histology slide with dimensions of 1 inch x 3 inches; for mounting samples to be imaged on Fusion microscope
This is coverslip 1
This is also coverslip 1
- TMA
- Tissue Micro Array, an embedded set of multiple samples
- If possible, include a clear fiducial in the array, especially when samples may be difficult to differentiate by eye
This layout shows sample cores, but a TMA can be any set of multiple samples on a single coverslip or slide
Acceptable Samples
- Formalin-fixed, paraffin embedded (FFPE) tissue blocks.
- Formalin-fixed, paraffin embedded (FFPE) tissue sections (3-7 microns) on coverslips or slides.
Please use standard fixation methods to preserve integrity. Fixation with 10% neutral-buffered formalin for 6-72 hours is recommended.
- Flash or fresh frozen tissue samples in OCT blocks.